MBEM™ Growth Medium for tumor cell cultures
MBEM™ is optimized for ex vivo culture of tumor cells from solid human cancers
MBEM™ provides a nutritionally complete growth medium for a variety of human cell types without the need for additional growth supplements. This allows ex vivo culture of epithelial, endothelial and mesenchymal origin cells without artificial growth stimulatory factors or small molecule inhibitors of specific signaling pathways. Within the development of the media composition, growth supporting characteristics of the MBEM medium was assessed with primary tumor cell samples from over 100 donors representing 40 different human cancers, including some of the most rare cancer types known. Thus MBEM is optimal for projects where the growth conditions of the cells of interest are not fully known. For cryopreservation and live cell biobanking of tumor cell samples the MBEM medium can be used with the common cryprotective reagents and cell freezing protocols.
MBEM™ maintains lineage heterogeneity of primary tumor samples
The formulation of MBEM is optimized to support the growth of maximally diverse panel of human cell types. Unlike many other commercial cell culture medium the formulation of MBEM has been shown to maintain lineage heterogeneity in primary tumor culture for excess of 50 passages without selective enrichment or depletion of a particular lineage.
MBEM™ is shown to preserve differentation state heterogeneity
Human solid tumors consists of a complex mixture of many distinct cell types, e.g., epithelial, myoepithelial, mesenchymal, endothelial and immune cells. With use of fresh tumor samples for ex vivo cell biological research it is important that the primary cultures also represent this intra tumor cellular heterogeneity. Choosing the right cell culture medium is thus critical for establishing representative primary cell cultures. MBEM medium has been shown to maintain the original intra tumor lineage heterogeneity without enrichment of a particular sub-cell population in long term cultures. The images show immunofluorescence assessment of growth of specific sub-cell lineages from a tongue cancer biopsy following two-week culture in different media. Immunofluorescence staining of tumor representative markers cytokeratin-14 and cytokeratin-19 was used to distinguish myoepithelial (shown in green) and epithelial cells (shown in red), respectively.
MBEM™ increases the success rate of creating representative cancer cell cultures
Images below show immunophenotypic comparison of primary cell cultures established in parallel from a tongue cancer biopsy into different cell culture media. Flow cytometry analysis of CD24 and CD44 lineage markers was used to assess growth of stromal and epithelial cells in the different media following two weeks of ex vivo propagation. Tumor cells were maintained in classical serum supplemented RPMI medium, a commercial cancer cell culture medium, PTCC and Misvik Biology's MBEM™ medium. Over 90% of the cells growing in the MBEM were positive for the epithelial lineage marker CD24 and less than 10% were positive for the stromal lineage marker CD44.